Influence of Whole Body X-irradiation on the Liver and Possible Protective Effect by the Compatible Solute Ectoine in a Mouse Model
Sarhan D. A.,
Shehata R. R.,
Kandil Y. I.,
Kotb M. A.,
El-Bassiouni E. A.
Issue:
Volume 4, Issue 3, June 2016
Pages:
16-21
Received:
14 June 2016
Accepted:
27 June 2016
Published:
13 July 2016
Abstract: Exposure of mammals to ionizing radiation leads to the development of a complex dose-dependent series of physiological and pathological changes. The present study was undertaken to investigate the effect of whole body x-irradiation of male mice with a single w dose of 6 Gy on the levels of some cytokines and parameters of oxidative stress, after one and seven days, in the liver. The possible protective effect of the compatible solute ectoine, which is known to reduce cell stress effects on the molecular level, was evaluated. The x-irradiated mice showed elevated interleukins in the liver. The levels of both the pro-inflammatory (IL-1β and IL-6) and the anti-inflammatory (IL-10) interleukins reached several times the control value with quantitative differences. The disturbance by low-dose x-irradiation also affected PGE2. Malondialdehyde, used as indication of oxidative stress, was elevated in the livers of x-irradiated mice. Although, there were no statistically significant differences in the results of total glutathione among the irradiated mice as compared to control values, there were differences among the values of the reduced and oxidized forms. The calculated redox potentials, showed some variations in the different groups, but were all within the values indicating that the cells are in the proliferative phase and far from the apoptotic phase. Treatment with ectoine modulated all the tested biochemical effects induced by x-irradiation in the liver. All the tested parameters had a tendency to go back to near control values.
Abstract: Exposure of mammals to ionizing radiation leads to the development of a complex dose-dependent series of physiological and pathological changes. The present study was undertaken to investigate the effect of whole body x-irradiation of male mice with a single w dose of 6 Gy on the levels of some cytokines and parameters of oxidative stress, after on...
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