Effects of Extremely Low Frequency Magnetic Field on the Secondary Structures of β-Amyloid and Human Serum Albumin
Saqer Mohamad Darwish,
Husain Rashad Alsamamra,
Sawsan Eid Abusharkh,
Imtiaz Mohammed Khalid,
Rania Abdeljalil Alfaqeh,
Musa Mahmoud Abuteir
Issue:
Volume 5, Issue 6, December 2017
Pages:
89-103
Received:
28 November 2017
Accepted:
12 December 2017
Published:
10 January 2018
Abstract: Human serum albumin and β-amyloid were exposed to extremely low frequency (ELF) magnetic field of 1.5 mT intensity and 50 Hz frequency. The effects of exposure were investigated in the mid-infrared region by means of Fourier self-deconvolution spectroscopic analysis. The experimental results suggest that exposure to the ELF magnetic field has reversible effects on the out of phase combination of N–H in plane bending and C–N stretching vibrations of the secondary structures of the two proteins. The exposure of β-amyloid and human serum albumin to ELF magnetic field affected the absorption spectra of the vibration bands by changes in peak positions for the amide II bands and changes of intensities in most of the bands in the amide I and amide II regions. In the fingerprint region, the most sensitive vibrations to the magnetic field are found to be in the (720-600) cm-I range. After removing the magnetic field, it took the vibration bands more than 10 minutes of a gradual change toward returning to their original spectra, obtained before the exposure. It is suggested that hydrogen bonds can alter the frequency of a stretching vibration depending on the increase or decrease of strain on the vibrations.
Abstract: Human serum albumin and β-amyloid were exposed to extremely low frequency (ELF) magnetic field of 1.5 mT intensity and 50 Hz frequency. The effects of exposure were investigated in the mid-infrared region by means of Fourier self-deconvolution spectroscopic analysis. The experimental results suggest that exposure to the ELF magnetic field has rever...
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Molecular Phylogenetic Study on Pseudomonas stutzeri Isolated from Currency Notes in Khartoum State, Sudan and Identified Via16s rRNA Gene Sequence Analysis
Mosab Yahya Al-Nour,
Malik Suliman Mohamed,
Noha Ahmed Abd Alfadil,
Hisham Nouraldayem Altayb,
Salah-Eldin Gumaa El-lzaki,
Mohamed Ahmed Hassan
Issue:
Volume 5, Issue 6, December 2017
Pages:
104-111
Received:
28 November 2017
Accepted:
19 December 2017
Published:
17 January 2018
Abstract: Pseudomonas stutzeri is a valuable bacteria for understanding of the taxonomical and the phylogenetic relationships. The study of genetic relationships between organisms or genes is carried out by molecular phylogeny. We aim to study the relationships according to16S rRNA gene sequences between our samples and the closest strains from other countries over the world. To our knowledge, the phylogenetic studies between strains from Sudan with strains from other countries were not done before. A total of 140 currency notes in different denominations were collected randomly from several locations including hospitals, food sellers and transporters. From the collected notes, a total of 135 bacterial colonies were isolated and from them 14 isolates were identified as a Pseudomonas stutzeri. In the study, streaking plate method was used for the isolation of pure bacterial culture, Chelex 100 method was used for DNA extraction, conventional PCR was used for amplification of the targeted gene, agarose gel electrophoresis and various bioinformatics tools were used for nucleotide sequence analysis. The PCR products were sent for Macrogen Company-Netherlands for purification and nucleotide sequencing. After sequencing 3 samples were noisy, hence they were excluded. According to phylogenetic analysis, we found that the samples were closely related to strains from south-east Asia (Indonesia), east Asia (China), south-central Asia (Bangladesh), south Asia (India), north Africa (Tunisia) and south Europe (Italy and Greece). Despite the samples were from the same source (currency notes), we found that there is broad sequence variation between them.
Abstract: Pseudomonas stutzeri is a valuable bacteria for understanding of the taxonomical and the phylogenetic relationships. The study of genetic relationships between organisms or genes is carried out by molecular phylogeny. We aim to study the relationships according to16S rRNA gene sequences between our samples and the closest strains from other countri...
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