Effect of Water Submergence on the Characteristics of Bituminous Mixes Using Reclaimed Asphalt Pavement
Md. Akhtar Hossain,
Md. Harun Ar Rashid,
Md. Mamunur Rashid
Issue:
Volume 6, Issue 1, June 2018
Pages:
1-6
Received:
18 December 2017
Accepted:
4 January 2018
Published:
19 January 2018
Abstract: Now a-days, the cost of asphalt pavement materials have been increasing excessively, which led to find alternative materials which must be cheaper than that of asphalt pavement materials. In addition, more concerns are pointed to reserving natural resources and reducing environmental impacts of using virgin asphalt binders, thus more attention is focused on the use of recycled materials in pavement designs. The main purpose of this study is to investigate the effect of water on the use of reclaimed asphalt pavement materials in bituminous mix and to determine the optimum percentage of reclaimed asphalt pavement materials with virgin pavement materials and optimum days of water submergence according to the Marshall Mix design criteria based on medium traffic condition. To achieve the objectives of this study the basic properties tests were performed on the studied materials and then Marshall Test was conducted on asphalt mixtures with different percentages of reclaimed asphalt pavement materials with optimum bitumen content determined for 100% fresh aggregate. The different percentages of reclaimed asphalt pavement material in asphalt mixtures are 0%, 10%, 20%, 30% and 40%. Marshall Criteria was satisfied up to 20%. Then the specimen prepared with 20% reclaimed asphalt pavement material was submerged in water at 0, 5, 10, 15 and 20 days. Optimum days of water submergence was 15 days on the basis of Marshall mix design criteria.
Abstract: Now a-days, the cost of asphalt pavement materials have been increasing excessively, which led to find alternative materials which must be cheaper than that of asphalt pavement materials. In addition, more concerns are pointed to reserving natural resources and reducing environmental impacts of using virgin asphalt binders, thus more attention is f...
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A Pipeline for Markers Selection Using Restriction Site Associated DNA Sequencing (RADSeq)
Issue:
Volume 6, Issue 1, June 2018
Pages:
7-16
Received:
19 October 2017
Accepted:
27 December 2017
Published:
20 January 2018
Abstract: Motivation: The discovery and assessment genetic variants for Next Generation Sequencing (NGS), including Restriction site Associated DNA sequencing (RADSeq), is an important task in bioinformatics and comparative genetics. The genetic variants can be single-nucleotide polymorphisms (SNPs), insertions and deletions (Indels) when compared to a reference genome. Usually, the short reads are aligned to a reference genome at first using NGS alignment software, such as the Burrows- Wheeler Aligner (BWA). The alignment is usually stored into a BAM file, a binary format of standard SAM (Sequence Alignment/Map) protocol. Then analysis software, such as Genome analysis Toolkit (GATK) or SAMTools [30] [31], together with scripts written in R programming language, could provide an efficient solution for calling variants. We focused on RADSeq-based marker selection for Arabidopsis thaliana. RADSeq consists short reads that do not cover the whole reference genome. Finally, SNPs as output in Variant Call Format (VCF) have been visualized by Integrative Genomics Viewer (IGV) software. We found that the visualization of SNPs and Indels is helpful and provides us with valuable insights on marker selection. We found that applying Chi-Square test for all target genotypes, which are homozygous reference 0/0, heterozygous variants 0/1 and homozygous variants 1/1, to test Hardy-Weinberg Equilibrium (HWE) in order to reduce false positive rate significantly and we showed that our pipeline is efficient in RADSeq-based marker selection.
Abstract: Motivation: The discovery and assessment genetic variants for Next Generation Sequencing (NGS), including Restriction site Associated DNA sequencing (RADSeq), is an important task in bioinformatics and comparative genetics. The genetic variants can be single-nucleotide polymorphisms (SNPs), insertions and deletions (Indels) when compared to a refer...
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