Studies of Thyroid Hormones (Propylthiouracil and L-Thyroxine) Interaction with Human Serum Albumin-Spectroscopic Approach
Israa Ismail Abu Katteh,
Husain Rashad Alsamamra,
Musa Mahmoud Abu Teir
Issue:
Volume 11, Issue 1, June 2023
Pages:
1-16
Received:
25 February 2023
Accepted:
21 March 2023
Published:
31 March 2023
Abstract: In this work, UV-absorption, fluorescence spectroscopy and Fourier transform infrared (FTIR) spectroscopy were used to investigate the relationship between Propylthiouracil and L-Thyroxine with human serum albumin. The binding constants of propylthiouracil and L-Thyroxine have been determined of both UV-absorption and fluorescence spectroscopy. The binding Constants values measured at 293k are 1.659×103 M-1 for propylthiouracil and 1.013×104 M-1 for L-Thyroxine. The constant values of the Stern–Volmer quenching were determined to be 2.144×103 L mol-1 for propylthiouracil and 1.937×103 L mol-1 L-for Thyroxine. The UV-absorption intensity of HSA- hormones complexes has increased with increasing of propylthiouracil and L-Thyroxine concentration. With the rise in propylthiouracil and L-thyroxine concentrations, the fluorescence results indicate a decrease in HSA- hormone emission intensity. To determine the effects of protein secondary structure and hormone binding mechanisms, we have used FTIR spectroscopy with Fourier self-deconvolution technique and second derivative resolution enhancement, as well as curve-fitting methods for the investigation of the amide I, II, and III regions. The peak positions within the three amide regions (amide I, amide II and amide III) were allocated and any results were explored due to changes in concentration. Due to variations in hormone concentrations, the measurements of the FTIR spectra indicate a difference in the intensity of absorption bands.. FTIR Spectra measurements reflect a difference in the strength of the absorption bands due to changes in hormone concentrations.
Abstract: In this work, UV-absorption, fluorescence spectroscopy and Fourier transform infrared (FTIR) spectroscopy were used to investigate the relationship between Propylthiouracil and L-Thyroxine with human serum albumin. The binding constants of propylthiouracil and L-Thyroxine have been determined of both UV-absorption and fluorescence spectroscopy. The...
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Radiolabeling with Technetium 99m for the Diagnosis of Coronary Heart Disease in Senegal
Papa Mady Sy,
Alphonse Rodrigue Djiboune,
Sidy Mouhamed Dieng,
Boucar Ndong,
El Hadji Amadou Lamine Bathily,
Ousseynou Diop,
Mamadou Soumboundou,
Louis Augustin Diaga Diouf,
Gora Mbaye,
Mamadou Mbodj,
Mounibé Diarra,
Sylvie Seck Gassama
Issue:
Volume 11, Issue 1, June 2023
Pages:
17-24
Received:
17 April 2023
Accepted:
9 May 2023
Published:
24 May 2023
Abstract: Coronary heart disease is the leading cause of death in industrialized countries. In Africa, its prevalence is increasing in line with the westernization of lifestyles, the improvement of socio-economic conditions and the increase in life expectancy. Therefore, myocardial perfusion scintigraphy presents itself as an effective imaging technique allowing improved prevention, diagnosis and therapeutic management and monitoring of patients at risk. Our present work has exclusively focused on the early diagnosis of patients at risk in the nuclear medicine department of the Grand Yoff General Hospital (HOGIP). Our work consisted of administering a radiopharmaceutical to a patient and monitoring its fate in the body by external detection of gamma radiation using a Mediso Nucline TM gamma camera. We used vectors like sestamibi and tetrofosmin. These vectors have the particularity of having an affinity and a tropism for the myocardial tissue. These two vectors were labeled with technetium 99m (radiotracer). Indeed, these radioactive tracers attach themselves to the organ to be studied from where they emit gamma radiation captured by a gamma camera which detects the signal from this organ and reconstructs a computerized static or dynamic image. This made it possible to make a diagnosis of ischemia or myocardial necrosis in 57% of cases in women and 82.5% of cases in men in a sample of 45 patients. Thanks to the qualitative visual interpretation of the distribution of the tracer, a diagnostic aid was provided by the semi-quantitative analysis provided by the study of the polar maps (bull's eyes). This myocardial perfusion scintigraphy can be a valuable diagnostic aid, especially in our countries where income is limited.
Abstract: Coronary heart disease is the leading cause of death in industrialized countries. In Africa, its prevalence is increasing in line with the westernization of lifestyles, the improvement of socio-economic conditions and the increase in life expectancy. Therefore, myocardial perfusion scintigraphy presents itself as an effective imaging technique allo...
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