Volume 8, Issue 2, December 2020, Page: 76-81
Determination of Endogenous Radioresistance Biomarkers on HeLa Cells
Mamadou Soumboundou, Laboratory of Biophysics, Department of Biology and Functional Explorations, Thies University, Thies, Senegal; Diagnostic Laboratory, Children Hospital of Diamniadio, Dakar, Senegal; Laboratory of Pharmaceutical Physics, Pharmacy Department, University Cheikh Anta Diop of Dakar (UCAD), Dakar, Senegal
Marie Thérèse Aloy, Molecular and Cellular Radiobiology Laboratory, University of Claude Bernard, Villeurbanne, France
Macoura Gadji, Service of Hematology, National Centre of Blood Transfusion (CNTS), Department of Applied Biological and Pharmaceutical Sciences, Faculty of Medicine, Pharmacy and Odontology (FMPO), University Cheikh Anta Diop of Dakar (UCAD), Dakar, Senegal; Research Institute in Oncology & Haematology (RIOH), Cancer Care Manitoba (CCMB), Department of Physiology & Pathophysiology, University of Manitoba (UFM), Winnipeg, Manitoba, Canada
Celine Malesys, Molecular and Cellular Radiobiology Laboratory, University of Claude Bernard, Villeurbanne, France
Gora Mbaye, Laboratory of Pharmaceutical Physics, Pharmacy Department, University Cheikh Anta Diop of Dakar (UCAD), Dakar, Senegal
Ahmadou Dem, Cancer Institute, Department of surgery, University Cheikh Anta Diop of Dakar (UCAD), Dakar, Senegal
Mounibe Diarra, Laboratory of Pharmaceutical Physics, Pharmacy Department, University Cheikh Anta Diop of Dakar (UCAD), Dakar, Senegal
Claire Rodriguez-Lafrasse, Molecular and Cellular Radiobiology Laboratory, University of Claude Bernard, Villeurbanne, France
Received: Oct. 18, 2019;       Accepted: Nov. 27, 2019;       Published: Dec. 28, 2020
DOI: 10.11648/j.ejb.20200802.18      View  40      Downloads  14
The Genes such as carbonic anhydrase IX (CAIX), hypoxia inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) have been suggested as hypoxic biomarkers in cancer. Indeed, these endogenous biomarkers have been shown to have stronger prognostic value response after treatment by irradiation. However, inconsistent results suggest that factors other than oxygen influence their expression. This present study deciphers the level of expression of different radioresistance biomarkers in both normoxia and hypoxia conditions followed by irradiation of human ovarian tumor cell lines (uterine cervix squamous cell carcinoma (HeLa). HeLa cells were submitted to hypoxia (1% O2) conditions in a Thermo Scientific Heracell i CO2 incubator. The cells were subjected to two doses 4-10 Gy irradiation and re-incubate in their starting conditions for 4 hours, then fixed in 4% paraformaldehyde for 20 min. Protein expressions were assessed by immunocytochemistry staining and fluorescent images were captured by a Axio Imager Z1 fluorescence microscope with oil immersion lens at 63× magnification. In normoxia conditions there was no modification of the level of expression of the CAIX after irradiation. However, an increasing expression level of VEGF was noted. The level of expression of HIF-1 in normoxia was low compared to the other two proteins (CAIX and VEGF). Hypoxia conditions at 2% resulted in a low expression of CAIX and VEGF before and after irradiation at 10 Gy in HeLa cells. HIF-1 had a maximum expression level compared to CAIX and VEGF at 2% oxygen after irradiation in HeLa cells. As tumor hypoxia occurs in a deprived microenvironment, other environmental factors such as irradiation might interact with the effect of low oxygen concentration on gene expression. This study shows that irradiation of HeLa cells has a profound influence on the oxygen dependent induction of certain endogenous hypoxic markers as HIF-1, CAIX, and VEGF.
Radioresistance, Biomarkers, Hypoxia, Normoxia, DNA Repair HELA Cells
To cite this article
Mamadou Soumboundou, Marie Thérèse Aloy, Macoura Gadji, Celine Malesys, Gora Mbaye, Ahmadou Dem, Mounibe Diarra, Claire Rodriguez-Lafrasse, Determination of Endogenous Radioresistance Biomarkers on HeLa Cells, European Journal of Biophysics. Vol. 8, No. 2, 2020, pp. 76-81. doi: 10.11648/j.ejb.20200802.18
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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